Research Article

An investigation on the in vitro wound healing activity and phytochemical composition of Hypericum pseudolaeve Robson growing in Turkey

10.4274/tjps.80037

  • Bahar Kaptaner İğci
  • Zeki Aytaç

Received Date: 22.08.2019 Accepted Date: 31.10.2019 Turk J Pharm Sci 0;0(0):0-0 [e-Pub]

INTRODUCTION:

This study was aimed to investigate the in vitro wound healing effects of the methanolic and aqueous extracts of Hypericum pseudolaeve Robson obtained by two different methods as well as its cytotoxicity, antioxidant activity and selected phytochemical constituents.

METHODS:

Total phenolic and flavonoid contents were measured using spectrophotometry based methods. Cytotoxic effects of the extracts on L929 mouse fibroblast cells were evaluated by MTT assay. Moreover, migration and spreading of the treated fibroblast cells were assessed by cell scratch assay as an in vitro wound healing model. In addition, the chemical content of the species was determined by High Performance Liquid Chromatography (HPLC).

RESULTS:

The results of cytotoxicity assay indicated that the methanolic and aqueous extract did not have any cytotoxic effect on fibroblast cells at concentrations up to 500 µg/mL. Fibroblast migration was significantly increased by 62 µg/mL concentration of the aqueous extracts compared to negative control. Extracts showed good antioxidant activity and 16 phytochemical compounds were detected by HPLC, with the highest amount for epicatechin.

DISCUSSION AND CONCLUSION:

Our results showed that H. pseudolaeve extracts have wound healing potential and contain several important antioxidant phenolic compounds. This species deserve further investigation aiming to isolate and identify the active compounds.

Keywords: Wound healing, plant extract, antioxidant, phenolics, HPLC