Original Article

Antibacterial, antifungal and antioxidant activity of Cleome coluteoides: An In Vitro Comparative Study between Leaves, Stems, and Flowers


  • Parastoo Zarghami Moghaddam
  • Ameneh Mohammadi
  • Paiman Alesheikh
  • Peyman Feyzi
  • Ali Haghbin
  • Samaneh Mollazadeh
  • Zahra Sabeti
  • Ailar Nakhlband
  • Jamal Kasaian

Turk J Pharm Sci 0;0(0):0-0 [e-Pub]


Cleome coluteoides belongs to Capparidaceae family and has been used in folk medicine for a long time. The aim of our research was measuring the antioxidant, antibacterial and antifungal activities of C. coluteoides.

Materials and Methods:

different parts of plant were extracted by various solvents (ethyl acetate, methanol and dichloromethane). Antibacterial and antifungal activities were assayed by disk and well diffusion methods. The antioxidant activity was screened by DPPH and FRAP methods.


The results showed that Gram negative bacteria and fungus were resistant to various plant extracts. Flower extract of C. coluteoides had the highest inhibition effects against all of tested gram positive bacteria. Also, Bacillus cereus was the most sensitive bacteria with inhibition zone of 18 mm. In antioxidant activity assay, different extracts of C. coluteoides exhibited various antioxidant activities due to the physical and chemical properties of solvent. In a way that, methanol extract of leaves showed the highest DPPH radical scavenging activity at various concentrations ranged from 5-160 mg.ml-1. Among all tested extracts, the highest level of phenolics was observed in the methanol extract of flower.

Discussion and Conclusion:

This study demonstrates that different extracts from various parts of C. coluteoides are different in their properties, so a proper solvent would be used to extract maximum amounts of antioxidant and antibacterial components from a typical plant material.

Keywords: Cleome coluteoides, Antibacterial, Antifungal, Antioxidant