Original Article

Cytotoxic Activity of Sesquiterpenoids Isolated from Endemic Ferula tenuissima Hub.-Mor & Peşmen

10.4274/tjps.galenos.2018.23356

  • Fadime AYDOĞAN
  • Şura BAYKAN
  • Bilge DEBELEÇ BÜTÜNER

Received Date: 11.07.2018 Accepted Date: 06.09.2018 Turk J Pharm Sci 2019;16(4):476-480

Objectives:

This was a phytochemical study of endemic Ferula tenuissima roots and determined the cytotoxic activity of pure compounds on PC-3.

Materials and Methods:

Air-dried and powdered roots of F. tenuissima (1 kg) were extracted consecutively with n-hexane, chloroform (CHCl3), and methanol (MeOH) (3×2 L, each) by sonication at 30°C for 24 h. The extracts were then filtered. The solvents were separately evaporated under reduced pressure to dryness. The compounds were isolated by chromatographic methods and their structures were determined by spectral methods (1D and 2D NMR and LC-MS). The compounds were tested for their cytotoxic activities versus the PC-3 cell line by WST assay.

Results:

A phytochemical investigation of the dried roots of endemic F. tenuissima was performed and three sesquiterpene esters were isolated. The daucane-type sesquiterpenes teferidin, ferutinin, and elaeochytrin-A were identified. In the bioactivity study, ferutinin exhibited the highest cytotoxic activity, with an IC50 value of 19.7 μM.

Conclusion:

The results indicate that the main compounds of F. tenuissima roots are daucane sesquiterpenes and ferutinin has a potential effect on PC-3 cells.

Keywords: Ferula tenuissima, daucane sesquiterpene esters, cytotoxicity, prostate cancer

INTRODUCTION

In recent years, cancer has become the main cause of public health problems and the second leading cause of death in the world despite advanced imaging and molecular diagnostic techniques.1 Prostate cancer is the most common cancer in men and is the second leading cause of cancer deaths in the United States after lung cancer.1 Today, most cancer drugs used as cytotoxic agents are obtained directly from natural products like plants, marine organisms, and microorganisms or indirectly by the semisynthesis of molecules from these sources. As a result, cancer research on natural products is expanding.2

The genus Ferula L. of the family Apiaceae (Umbelliferae) is represented by about 185 species worldwide and 23 taxa in Turkey.3 Several species, such as the roots of Ferula gummosa and Ferula asafeotida, have been used in folk medicine as an antidote in poisonings and as an aphrodisiac, antimicrobial, expectorant, and antihemorrhoidal, as well as to treat stomachache, colitis in infants, asthma, and urinary tract disorders.4 Monoterpenes, sesquiterpenes (especially daucane-, humulane-, and guaiane-type sesquiterpene esters and sesquiterpene lactones), and coumarins were found to be the main constituents of the genus Ferula by phytochemical studies.5-8 Phenylpropanoid, sulfur-containing derivates, and triterpenes and their glycosides were also reported.9-11 Recent pharmacological research has demonstrated that different extracts of Ferula species contain sesquiterpene derivatives that have in particular proven to be cytotoxic on several cancer cell lines.2,12 In addition, the extracts have been reported to have antimicrobial, anthelmintic, anticonvulsant, antispasmodic, antihyperglycemic, antihyperlipidemic, and antioxidant activities.13-18


EXPERIMENTAL


Plant material

The roots of Ferula tenuissima Hub.-Mor & Peşmen were collected in the Yarpuz Region, Osmaniye, Turkey (940 m) in June 2013. The whole plant was identified by Assoc. Prof. Serdar Gokhan Senol from the Section of Botany, Department of Biology, Faculty of Science, Ege University. A voucher specimen (IZEF 6046) was deposited in the Herbarium of Ege University, Faculty of Pharmacy, İzmir, Turkey (www.izef.ege.edu.tr).


Extraction and isolation

Air-dried and powdered roots of F. tenuissima (1 kg) were extracted consecutively with n-hexane, chloroform (CHCl3), and methanol (MeOH) (3×2 L, each) by sonication at 30°C for 24 h. The extracts were then filtered. The solvents were separately evaporated under reduced pressure to dryness. Yields were 44.31 g, 9.90 g, and 45.89 g, respectively. Next 9.27 g of the CHCl3 extract was submitted to silica gel column chromatography and eluted consecutively with n-hexane: EtOAc gradient (100:0-0:100, v/v, 10% decreasing polarity, each 500 mL), EtOAc: acetone (100:0-0:100, v/v, 10% decreasing polarity), and then acetone: MeOH (100:0-0:100, v/v, 10% decreasing polarity) to give 15 fractions, named A-O, and monitored by thin-layer chromatography (TLC). Based on the TLC profiles 3 fractions, i.e. fractions B (210 mg), E (233 mg), and G (200 mg), were selected for further purification. Fraction B was chromatographed over a silica gel column (150 g) with n-hexane: EtOAc (100:0-87.5:12.5, with 2.5% increasing polarity) to afford five fractions (B1-B5). Fraction B3 (83 mg) was rechromatographed over a silica gel column (75 g) with n-hexane: EtOAc (100:0-90:10, 2% decreasing polarity) to yield compound 1 (40 mg) purely. Then 33 mg of fraction E was further purified by preparative TLC (n-hexane:EtOAc, 80:20, silica gel) and isolated and yielded 17 mg of compound 3. Fraction G was submitted to silica gel column chromatography, eluted with n-hexane: EtOAc (95:5-50:50; 5% increasing polarity) solvent, and yielded compound 2 (33 mg).


Cytotoxicity assay and cells

Cell toxicity was analyzed by using WST-1 according to the manufacturer’s protocol. PC-3 and RWPE-1 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). PC3 cells were propagated using DMEM F-12 supplemented with 5% FBS, L-glutamine (2 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL), while RWPE-1 cells were propagated in keratinocyte growth medium supplemented with bovine pituitary extract and 5 µM EGF at 37°C with 5% CO2. Molecules were dissolved in DMSO and treatments were done so that DMSO volume would not exceed 0.5% of the culture media volume. Control cells were treated with the same volume of DMSO used during the molecule treatments. PC-3 “(8x103)” and RWPE-1 cells “(104)” were seeded and grown in 96-well plates and incubated for 24 h. Molecule treatments were performed for 48 h and WST-1 cell proliferation reagent (roche cat no: 05015944001) was used as recommended. Briefly, WST-1 (1:10 final dilution) was added to the cells at the end of treatments, and the cells were incubated for an additional 3 h. At the end of the incubation, absorbance was measured at 450 and 690 nm using a SpectraMaxPlus 384 spectrophotometer (Molecular Devices). IC50 concentrations of the molecules were calculated through nonlinear regression analysis in GraphPad Prism 6. All experiments were performed in triplicate. Doxorubicin was used as the positive control.


Chemicals and other materials

Mass spectra (Thermo-Scientific TSQ Quantum Access Max LC-MS/MS, ESI). Nuclear magnetic resonance (NMR) spectra were recorded on a Varian Oxford AS400 and a Bruker DRX-500. The chemical shifts were measured relative to the residual solvent peak and are expressed in δ (ppm) and the coupling constants (J) are reported in Hertz (Hz). Column chromatography was carried out on silica gel 60 (40-63 mm, Merck), Sephadex LH-20 (GE Healthcare), and Micropore RP-18 (25-40 mm, Merck) using analytical grade purity solvents (Merck and Sigma). TLC analyses were carried out on silica gel 60 F254 and RP-18 F254s (Merck) precoated aluminum plates. Compounds were detected by UV (244-366 nm) and 10% vanillin ethanol solution/H2SO4 reagent followed by heating at 105°C for 1-2 min.


RESULTS AND DISCUSSION

The powdered roots of F. tenuissima were extracted consecutively with n-hexane, CHCl3, and MeOH (3×2 L, each) by sonication at 30°C for 24 h. The CHCl3-soluble fraction was subjected to repeated column chromatography (CC) to afford three known compounds (see Figure 1). All of them were daucane-type sesquiterpenoids; their structures were established by NMR and MS and by comparison with published data. Compounds 1 (teferidin),19 2 (ferutinin),19 and 3 (elaeochytrin-A)20 were also identified by comparison of their spectral data with those in the literature.19,20


Compound 1 (Teferidin)

4β-Hydroxy-6α-benzoyloxy-5α(H)-dauc-8-ene: Yellow residue, EI MS/MS, [M]+ at m/z=342.16 for C22H30O3; 1H, 13C NMR spectroscopic data, see Table 1.


Compound 2 (Ferutinin)

4β-Hydroxy-6α-(p-hydroxy benzoyloxy)-5α(H)-dauc-8-ene: Yellow residue, EI MS/MS [M+H]+ at m/z=359.06, [M+NH4]+ m/z=376.11, [M+Na]+ m/z=381.06, [M+K]+ m/z=397.03 for C22H30O4; 1H, 13C NMR spectroscopic data, see Table 1.


Compound 3 (Elaeochytrin-A)

4β-Hydroxy-6α-(o-amino benzoyloxy)-5α(H)-dauc-8-ene. Yellow residue, EI MS [M+Na]+ m/z=380.34 for C22H31NO3; 1H, 13C NMR spectroscopic data, see Table 1.

All compounds isolated from F. tenuissima were evaluated for their cytotoxic activity against the PC-3 cancer cell and normal prostate RWPE cell lines. The IC50 values of compounds that are active on at least one cell line at concentration are given in Table 2.


CONCLUSION

Mono-, di-, triesters of humulane, germacrane, eudesmane, and especially daucane-type sesquiterpenes and coumarin and lactone derivates are major components of the genus Ferula L.21 It was observed that the location of the double bond in the daucane ring affected activity, which was at positions 7-8, 8-9, and 9-10. Furthermore, the presence of hydroxyl groups at different positions on the daucane ring and the formation of mono-, di-, and tri-ester structures of this hydroxyl group, especially benzoic, angelic, cinnamic, and vanillic acid, increases the variability in biological activity.21 The isolated teferidin compound is jaeschkeanadiol benzoic acid ester isolated from F. tenuisecta roots for the first time in 1976.2 It has also been reported from F. hermonis, F. pallida, F. elaeochytris, F. rigidula, and F. jaeschkeana roots.21 Ferutinin was first described in 1973 by Saidkozev as jaeschkeanadiol p-hydroxy benzoic ester.23 It was isolated from different Ferula species previously.23 Elaeochytrin-A was first reported from F. elaeochytris roots.20

In our study, it was determined that all compounds were moderately effective on the PC-3 and RWPE-1 cell lines. The affinity of the compounds for RWPE-1 cells also indicated that the selectivity of the compound is not as high as expected.

In a previous study, elaeochytrin-A showed cytotoxic effects on K562R (imatinib-resistant) human chronic myeloid leukemia and DA1-3b/M2BCR-ABL (dasatinib-resistant) mouse leukemia cell lines at IC50 12.4 and 7.8 µM concentrations, respectively.20 In the same study, ferutinin showed cytotoxic activity at IC50 25.3 and 29.1 µM and teferidin at IC50 55.1 and 29.5 µM. When the molecular structures were examined, the double bond between C8 and C9 positions decreased cytotoxic activity.20 Ferutinin has been shown to have an antiproliferative effect on colon cancer cell lines of WiDr, COLO320-HSR, and LS-174T24 and to induce apoptosis and intracellular Ca+2 pathway in human Jurkat cells.25 Ferutinin showed ERα and ERβ agonist and antagonist receptor activity with improving sexual function in male and female rats.26 It has been found that the hydroxyl group at position 3 increases the estrogen-like effect of the presence of oxygen and the presence of electrophilic groups in the p-position of the benzene ring (hydroxyl, oxo, etc.).26 Prostate cancer formation, especially androgenic hormones, is the main cause of uncontrolled proliferation of cells. Ferutinin molecule studies suggest that both the effects on sexual function and the activity of in vitro cytotoxicity studies may be specific antagonist/agonist effects on androgen hormone receptors.26 As a result of our bioactivity studies on the PC-3 cell line with compounds isolated from F. tenuissima roots, the most active cytotoxic agent of ferutinin synthesis emerged (IC50: 19.69 µM), in addition to the potential phototherapeutic results of phytochemical and bioactivity studies of other genus-related species due to the biological activity of the root extracts of Ferula taxa and daucane-type sesquiterpenoids.


ACKNOWLEDGEMENTS

We thank Prof. Dr. Erdal Bedir, Assoc. Prof. Serdar Gokhan Senol, and Assoc. Prof. Bintuğ Öztürk for their support. This research project was supported by the Scientific Research Projects Directorate of Ege University (Project No: 13-ECZ-022).

Conflict of Interest: No conflict of interest was declared by the authors.

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