Original Article

Determination of Hispidulin in the flowers of Inula viscosa (L.) Aiton Using HPLC and HPTLC Methods

  • Alper GÖKBULUT

Received Date: 08.10.2015 Accepted Date: 17.12.2015 Turk J Pharm Sci 2016;13(2):159-166

The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quanti-fied by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatog-raphy (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented. Both methods provided a ra-pid and easy approach for determination of the biomarker hispidulin. In the present study HPTLC and HPLC profiles of Inula viscosa flowers were presented to detect and quantify the small flavonoid hispidulin. The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.

Keywords: Hispidulin, Inula viscosa, HPLC, HPTLC