Original Article

Development of a new approach for standardization of Centaurium Erythraea Rafn. herb by HPLC method

10.4274/tjps.71542

  • Svitlana M. Gubar
  • Anna S. Materiienko
  • Nataliia M. Smielova
  • Liana G. Budanova
  • Victoriya A. Georgiyants

Turk J Pharm Sci 0;0(0):0-0 [e-Pub]

INTRODUCTION:

Development a new, fully validated HPLC method for the quantitative analysis of secoiridoid glycosides by an active marker swertiamarin in the Centaurium erythraea herb. The article describes a new approach to the standardization of the Centaurium erythraea Rafn. herb and more specifically the development of a new validated HPLC method for the quantitative determination of secoiridoid glycosides by the active marker swertiamarin.

METHODS:

Swertiamarin quantitative determination has been estimated in an isocratic mode, on a Symmetry C18 column, using water and acetonitrile as solvents for the mobile phase.

RESULTS:

Validation characteristics of the developed method have shown that it is linear in the whole range of concentrations from 0.01 mg/ml to 0.05 mg/ml of swertiamarin. All validation characteristics meet the established acceptance criteria.

DISCUSSION AND CONCLUSION:

This method can be used in the standardization of raw materials, as well as in the analysis of medicinal products and dietary supplements, which include the Centaurium erythraea herb. The established chromatographic method has been successfully applied for the analysis of raw materials of the Centaurium erythraea Rafn. herb with the quantitative content determination of swertiamarin in the analyzed samples.

Keywords: common centaury herb, development, HPLC, swertiamarin, validation