Original Article

Effect of Combined Treatment of Levofloxacin and Metformin on the Diabetes Related Behavioral and Biochemical Alterations


  • Poonam Singh
  • Vaibhav Walia
  • Prabhakar Kumar Verma

Received Date: 02.09.2023 Accepted Date: 12.01.2024 Turk J Pharm Sci 0;0(0):0-0 [e-Pub]


The current experiment was conducted to investigate the combined effect of levofloxacin and metformin treatment on glucose level in blood, MDA, nitrite, level, anxiety of STZ+NAD induced diabetic rats.


In this study, wistar rats have been used. After receiving a single dose of STZ+NAD (45 mg/kg, i.p.+ 50 mg/kg, i.p.), the rats developed diabetes. Glucose levels in diabetic rats exceed 200 mg/dl (verified on the third day). Saline was administered to non-diabetic rats (controls). The diabetic rats were given alone metformin (50 mg/kg, p.o.), levofloxacin (30 mg/kg, i.p.), or metformin + levofloxacin for fourteen days. The blood sample was obtained after the 14th day of therapy, and the rats were given to behavioral parameter to determine locomotor-activity and anxiousness level. Blood plasma sample has been separately and used for determination of nitrite and malondialdehyde (MDA) level.


It was observed that the combined treatment of metformin and levofloxacin significant rise glucose in blood of diabetic rats as compares to diabetic control (P < 0.05) and alone metformin treated diabetic rats (P < 0.001) at day-3 and day-7. Further, combined treatment of metformin and levofloxacin significantly reduce time spend at center of OFT (P < 0.001), significantly reduce time spend and entry made in light chamber of LDT (P < 0.001), significantly rise time spend in open arm and reduce the time spent in closed arm of EPM (P < 0.001) as compare to alone metformin treated diabetic rats. Further, combined treatment of metformin and levofloxacin significant increase nitrite level, (P < 0.001) but reduce the MDA level in plasma as compare to metformin alone treated-diabetic rats (P < 0.001).


In conclusion, the present study suggested the combined treatments of levofloxacin and metformin may modulate the glucose level and anxiety-related activity.

Keywords: Diabetes, glucose, malondialdehyde, anxiety, levofloxacin.