Research Article

Evaluation of Methylation and Acetylation Profiles of Dinitroaniline Herbicides and Resveratrol on V79 Cell Line


  • Zehra Sarıgöl Kılıç

Received Date: 11.09.2019 Accepted Date: 31.10.2019 Turk J Pharm Sci 0;0(0):0-0 [e-Pub]


Herbicides are one of the most widely used pesticide compounds for plant growth control worldwide. Risk assessment of dinitroanilin derived herbicides, pendimethalin and trifluralin, is important for foodborne or other means of exposure. In this study, we aimed to evaluate the methylation and acetylation profiles of pendimethalin and trifluralin, which we have high levels of exposure in various ways. Furthermore, it was aimed to determine the protective effect of resveratrol, an antioxidant compound, against the possible toxic effects of these pesticides.


The effects of pendimethalin and trifluralin alone (25, 50, 100 µM) and in combination with resveratrol (100 µM) on DNA methyltransferase (DNMT) 1, 3a, 3b; histone deacetylase (HDAC)1 and HDAC3 gene expressions were evaluated by RT-PCR.


According to the results of the experiment, pendimethalin caused a significant decrease in DNMT and HDAC expressions at all concentrations, whereas HDAC1 and 3 expressions were increased at the concentration of 25 µM, when applied together with resveratrol. Additionally, there is no change in DNMT1 and 3b expression levels. Unlike pendimethalin, trifluralin increased DNMT1 expression in concentration dependent manner. While DNMT3a and DNMT3b expression levels increased significantly, HDAC1 and 3 expression levels did not change significantly. The expression levels of HDAC1 and HDAC3 increased at all concentrations of trifluralin combination with resveratrol. Also, DNMT levels increased at the concentrations of 50 and 100 µM.


According to the epigenetic gene expression results, pendimethalin and trifluralin may cause tissue function loss and chromosome damage as a result of direct effects on cell viability by causing expression level changes in all studied genes. Also, it can be concluded that the changes that occur in gene expressions may induce tumor development. Further studies are needed to elucidate the possible toxicity mechanisms of these herbicides considering the relationship between epigenetic changes and various diseases.

Keywords: pendimethalin, trifluralin, epigenetic, DNA methyltransferase, histon deacetylase