Abstract The detection and quantification of Lycorine in the total alkaloidal extracts prepared from aerial and underground parts of Galanthus nivalis ssp. cilicicus, G. gracilis, G. elwesii and G. plicatus ssp. byzantinus, collected during two different vegetation periods, were carried out using high perform-ance liquid chromatography (HPLC). An isocratic system with chloroform/methanol mobile phase was used for the detection and quantitative determination of lycorine. External standard calibration was used to quantify lycorine. Özet Vejetasyonunun iki farkli zamaninda toplanmis olan Galanthus nivalis ssp. cilicicus, G. gracilis, G. elwesii ve G. plicatus ssp. byzantinus’un topraküstü ve toprakalti kisimlarindan hazirlanan total alkaloit ekstrelerinde Lycorine’in tespiti ve miktar tayini yüksek basinçli sivi kromatografisi (YBSK) kullanilarak gerçeklestirilmistir. Lycorine’in tespiti ve miktar tayini için kloroform/metanol mobil fazli bir izokratik sistemden yararlanilmistir. Lycorine miktarinin tayininde harici standart kalibrasyon yöntemi kullanilmistir.
Keywords: Galanthus, Amaryllidaceae, Lycorine, HPLC