Original Article

Method Validation of Contact and ImmersionTLC-bioautography for Determination of Streptomycin Sulfate in Shrimp

10.4274/tjps.galenos.2019.59489

  • Febri ANNURYANTI
  • Iftitahatur ROSYIDAH
  • Asri DARMAWATI
  • Isnaeni Isnaeni

Turk J Pharm Sci 0;0(0):0-0 [e-Pub]

Contact and immersion Thin Layer Chromatography (TLC)-bioautography have been developed for identification and quantification of streptomycin sulfate in shrimp. The TLC of streptomycin sulfate standard solution has been carried out by using silica gel F254 and 7.5 % of KH2PO4 solution as stationary and a mobile phase respectively. Retardation factor (Rf) of the streptomycin sulfate standard was 0.51 and the selectivity of streptomycin sulfate was 4.1 with the presence of kanamycin sulfate in the shrimp. The bioautograpy was performed by Escherichia coli ATCC 8739 as a test bacterial. Limit of Detection (LOD) of streptomycin sulfate obtained by the contact and immersion TLC-bioautography were 0.24 μg and 0.16 μg, respectively. Both methods showed good linearity with the r-value more than 0.999 and the Vxo value less than 2%. Accuracy of the contact and immersion TLC-bioautography were done using standard addition method and the obtained percentage recovery were 86.93±1.60% and 96.42±0.65%, respectively. Coefficient variation of the contact and immersion TLCbioautography were 2.39±1.79% and 0.53±0.17 %, respectively. The immersion TLCbioautography was more sensitive with better recovery than contact TLCBioautography. In addition, immersion TLC-bioautography method was successfully employed for determination of streptomycin sulfate in shrimp.

Keywords: Streptomycin sulfate, contact TLC-bioautography, immersionTLCbioautography, shrimp