Two new simple, sensitive and rapid spectrophotometric methods were developed for the quantitative estimation of amisulpride (AMS) in bulk drug and pharmaceutical formulations (tablets). The methods are based on the reaction of amisulpride with sodium nitrite in acid medium to form diazonium salt, which is coupled with phloroglucinol (Method A) and resorcinol (Method B) to form azo dyes showing absorption maxima at 418.5 nm and 403.8 nm respectively. Beer's law is obeyed in the concentration range of 2-10 ug/mL of amisulpride for method A and 4-20 ug/mL of amisulpride for method B. The molar absorptivity and sandell’s sensitivity of AMS-phloroglucinol and AMS-resorcinoI are 1.8 x 104, 0.25 and 1.1 x 104, 0.0625 respectively. The optimum reaction conditions and other analytical parameters were evaluated. The methods were successfully extended to pharmaceutical preparations (tablets) containing amisulpride.
Keywords: Amisulpride, Spectrophotometric Determination, Phloroglucinol, Resorcinol