Original Article

VALIDATED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF LAMOTRIGINE IN HUMAN PLASMA AND SALIVA

  • Tuba İNCEÇAYIR
  • İlbeyi AĞABEYOĞLU

Received Date: 17.09.2009 Accepted Date: 22.01.2010 Turk J Pharm Sci 2010;7(3):213-224

A simple, sensitive and precise high performance liquid chromatographic assay for lamotrigine (LTG) is described. Good chromatographic separation was achieved using a 5 um ACE-5 CI8 reverse-phase column and a mobile phase consisting of methanol:acetonitrile: 0.01 M potassium phosphate monobasic (30:15:55, v/v/v) adjusted to pH 6.3 and delivered at a flow rate of 1 mL/min. Quantification was performed by measurement of the UV absorbance at a wavelenght of 304 nm. The method was linear in the range of 0.1-6.2 ug/mL with a coeffient of determination, (r2 = 0.999). The retention time of LTG was 6.8 and 6.2 min in plasma and saliva, respectively. The limit of quantification was 0.10 ug/mL. Recovery from plasma and saliva ranged from 97 to 98 and 96 to 105 %, respectively. Between-day and within day precision expressed as CV % in plasma and saliva were in the range of 0.26 to 6.8. The method was showed to be appropriate for pharmacokinetic (PK) studies and monitoring LTG in saliva and plasma samples.

Keywords: Lamotrigine, High performance liquid chromatography (HPLC), Plasma, Saliva.