Original Article

COMPARATIVE FREE RADICAL SCAVENGING CAPACITY OF THE SEED EXTRACTS OBTAINED FROM THE WHITE AND RED GRAPE BERRIES USED FOR WINE-MAKING IN TURKEY

  • Aysen ÇÖLKESEN
  • Ahmet AYDIN
  • Askin ISIMER
  • Ilkay ORHAN
  • Bilge SENER

Received Date: 09.02.2006 Accepted Date: 27.06.2006 Turk J Pharm Sci 2006;3(3):177-185

Abstract Free radical scavenger capacity of the polyphenolic fractions obtained from ethyl acetate extracts of the seeds of sixteen red or white-colored samples of grape (Vitis vinifera-Vitaceae) species cultivated in Turkey has been determined in this study. On this purpose, the scavenger activity of V. vinifera extracts was evaluated via stable free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) and superoxide radical (O2-.) generated by xanthine/xanthine oxidase (XOD) system. DPPH scavenger capacity of the extracts has been compared with the same concentrations of the known antioxidants such as BHT, vitamin C, gallic acid and quercetin. Vitamin C and gallic acid have been also used to compare O2-. scavenger capacity of the extracts. Most of the extracts displayed significant DPPH scavenger activity. Özet Bu çalismada, Türkiye’de kültürü yapilan 16 çesit kirmizi veya beyaz üzüm (Vitis vinifera L.-Vitaceae) örneklerinin tohumlarinin etilasetat ekstrelerinden elde edilen polifenolikfraksiyonlarin serbest radikal süpürücü etkileri tayin edilmistir. Bu amaçla, V. vinifera ekstrelerinin süpürücü aktivitesi, stabil serbest radikal 1,1-difenil-2-pikril hidrazil (DPPH) ve ksantin/ksantin oksidaz (XOD) sisteminin yarattigi süperoksit radikali (O2-.) araciligi ile degerlendirilmistir. Ekstrelerin DPPH süpürücü aktivitesi; BHT, vitamin C, gallik asit ve kersetin gibi bilinen antioksidanlarin ayni konsantrasyonlari ile karsilastirilmistir. Vitamin C ve gallik asit, ekstrelerin (O2-.) süpürücü kapasitesini karsilastirmak için de kullanilmistir. Ekstrelerin çogu dikkate deger DPPH süpürücü etki göstermistir.

Keywords: Free radical scavenging activity, Grape seed, Vitis vinifera, DPPH, O2-