The effect of zinc (Zn), iron (Fe) and copper (Cu) on phytoconstituent content and the potential antibacterial activity of three different leaf extracts of Alternanthera brasiliana (AB) against pathogenic bacteria, procured from two different states of India, was evaluated. Shade dried leaf samples were separately determined for Zn, Fe and Cu content through atomic absorption spectrophotometer (AAS). Further extraction was carried out using aqueous, methanol and mixture of aqueous and methanol (1:1) and microbioside potential were evaluated by agar well diffusion technique. Ampicillin (30μg/mL) was used as standard. The Minimum Inhibition Concentration (MIC) of all the extracts was carried out by serial dilution method and thereafter inhibitory microbial activity was carried out by agar plate method. The amount of Zn, Fe and Cu were higher in West Bengal sample. Furthermore, the concentration dependent (**P)
Keywords: Antimicrobial study, Agar diffusion technique, Elemental analysis, Extracts, Alternanthera brasiliana, MIC