In this study a total of 34 Bacillus cereus isolated from ice-cream samples in different pastry shops in Ankara, Turkey were evaluated for antibiotic resistance, which were collected from 34 samples of icecream. The isolates were identified by morphological, biochemical tests and confirmed by API 50 CUB. The susceptibility of 34 B. cereus to ampicillin (AMP), penicillin (P), tetracycline (TE), trimethoprim-sulphamethoxazole, erythromycin (E), ciprofloxacin (CIP), gentamicin (GM), and vancomycin (VA) were determined by both microdilution and disk diffusion methods. Among 34 B. cereus from isolated ice-cream samples no resistance were found to vancomycine,, gentamicin and ciprofloxacin, while they were found resistant to ampicillin (29.5%), penicillin (29.5%), and for trimethoprim-sulphamethoxazole (12%).. To tetracycline, another antibiotic investigated in this study, 6% resistance with microdilution method and 3% resistance with disc diffusion method was determined. To erythromycin, 3% resistance was determined with only disc diffusion method.
Keywords: Bacillus, Identification, Resistance