A simple and sensitive method for separation and determination of chlorogenic acid has been developed. Chlorogenic acid was separated using a Supelcosil LC 18 column (250 x 4.6 mm, 5 μm) by gradient elution at the flow rate of 1.2 mL/min. The composition of mobile phase consisted of ophosphoric acid, bidistilled water (0.2%) and acetonitrile. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for chlorogenic acid was between 0.7237-500 μg/mL. The method described was suitable for the determination of chlorogenic acid in the leaves, branches and fruits of Viburnum tinus L. and Viburnum orientale Pallas. It was observed that V.orientale fruit sample has the highest chlorogenic acid content (0.5069%), while V.tinus branches (0.0141%) have the lowest chlorogenic acid content as w/w (%) in our case. This study is the first application of High Performance Liquid Chromatography (HPLC) method to the determination of chlorogenic acid content of V. tinus and V. orientale in Turkey.
Keywords: Viburnum tinus, Viburnum orientale, HPLC, Chlorogenic acid