Original Article

IN VITRO SUSCEPTIBILITY OF Candida Spp. ISOLATED FROM CLINICAL SPECIMENS AGAINST SOME ANTIFUNGAL AGENTS

  • Berrin ÖZÇELIK
  • Neriman BALABAN
  • Sebahat AKSARAY
  • Salih CESUR
  • Fatma KAYNAK
  • Altan ÇAYIRLI

Received Date: 08.03.2006 Accepted Date: 16.03.2006 Turk J Pharm Sci 2006;3(1):1-6

Abstract We evaluated the in vitro activity of ketoconazole (KET), fluconazole (FLU), itraconazole (ITRA), amphotericin B (AmpB), flucytosine (FCU) in comparison to voriconazole (VOR) against Candida species isolated from blood cultures. The most common species of identified Candida were C. albicans (71), followed by C. parapsilosis (16), C. glabrata (12) C. tropicalis (9), C. kefyr (8) and one of C. lustaniae, C. spherical, C. sake, C. lambria respectively. NCCLS M 27-A method was used to evaluate the activity of KET, FLU, ITRA, AmpB, FCU and VOR. The MICs of the strains were evaluated by RPMI 1640 medium with microdilution method. There were no isolates of tested Candida spp. resistant to KET, ITRA, FCU, AmpB and VOR. Only two of C. albicans isolates were resistant to FLU (MIC; ≥32μg/ml). Intermediate resistant appeared in 17.5% isolates to KET and FLU, 8.3% isolates to ITRA. Among 120 Candida isolates were found highly susceptible to KET (MIC;≤8μg/ml) followed by VOR (MIC;≤0.03μg/ml), ITRA (MIC;≤0.125μg/ml) FCU (MIC;≤4μg/ml) AmpB (MIC; Özet Kan kültürlerinden izole edilen Candida türlerine karsi ketokonazol (KET), flukonazol (FLU), itrakonazol (ITRA), amfoterisin B (AmpB) ve flusitozin (FCU)’in in vitro duyarliliklari vorikonazol (VOR) ile karsilastirilarak degerlendirilmistir. En sik identifiye edilen Candida türleri sirasiyla C. albicans (71), C. parapsilosis (16), C. glabrata (12), C. tropicalis (9), C. kefyr (8) ve birer adet C. lustaniae, C. spherical, C. sake ve C. lambria’dir. KET, FLU, ITRA, Amp B, FCU ve VOR’un in vitro aktivitelerinin degerlendirilmesinde NCCLS M 27-A metodu kullanilmistir. MIK degerleri RPMI 1640 besiyeri kullanilarak mikrodilüsyon yöntemi ile deger-lendirilmistir. Test edilen Candida türleri; KET, ITRA, FCU, AmpB ve VOR’a dirençli degildir. Sadece iki C. albi-cans izolati FLU(MIC;≥32μg/ml)’e dirençlidir. Suslarin; KET ve FLU için %17.5, ITRA için %8.3’ünde orta duyarlilik görülmüstür. 120 Candida izolati KET (MIK;≤8μg/ml), VOR (MIK;≤0.03μg/m), ITRA (MIK;≤0.125μg/ml), FCU (MIK;≤4μg/ml), AmpB (MIK;

Keywords: Antifungal susceptibility; In vitro activity; Candida spp