ABSTRACT

In the present study, nine extracts of Centaurea tchihatcheffii 1weM screened for their in-vitro antiviral, antibacterial and antifungal activities. Antibacterial and antifimgal activities were evaluated against both standard and the isolated strains of Escherichia coli, Pseudomonas aeniginosa, Proteus mirabilis, Klebsiella pneumoniae, Acinetobacter baumannii. Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis as well as Candida albicans, Candida parapsilosis by broth microdilution method. Susceptibility testing was performed according to the Clinical and Laboratoiy Standards Institute (CLSI; formerly NCCLS). Furthermore, Herpes simplex virus Type-1 (HSV-1) and Parainfluenza-3 virus (PI-3 vims, were employed for antiviral assessment of the extracts by using Madin-Darby Bovine Kidney and Vero cell lines. Ampicilline, geiitamicin, ofloxacin, levofloxacin, ketoconazole, fluconazole, acyclovir and oseltamivir were used as the control agents. As our knowledge, this is the first report on these C. tchihatcheffii extracts that were evaluated for their antimicrobial activities. According to the data obtained, all the extracts appear to have antibacterial activity tested against standard Grain-negative and Gram-positive bacteria ranging from minimum inhibitory concentrations of 2 to 16 ug/mL, besides they have have antibacterial activity at 32->128 ug/mL concentrations to isolated strains. Notable activity was obseived with the extracts against C. albicans and C. parapsilosis fungi at 4 ug/mL and 8 ug/mL concentrations, respectively.The data showed that water-chloroform interpahse (U-1), chloroform (U-2), and ethyl acetate (U-3), have antiviral activity against both DNA (HSV-1) and RNA (PI-3) viruses.