ABSTRACT

The flavone hispidulin (4´,5,7-trihydroxy-6-methoxyflavone) in the flowers of Inula viscosa (L.) Aiton was quanti-fied by using High Performance Liquid Chromatography (HPLC), and also High Performance Thin-Layer Chromatog-raphy (HPTLC) fingerprint of the methanolic I. viscosa flower extract was presented. Both methods provided a ra-pid and easy approach for determination of the biomarker hispidulin. In the present study HPTLC and HPLC profiles of Inula viscosa flowers were presented to detect and quantify the small flavonoid hispidulin. The results reveal that I. viscosa flowers contain serious amount (0.151± 0.007 g/100 g dry weight) of hispidulin.