ABSTRACT

This research aims to examine the potential of the parasite Scurrula ferruginea (Roxb. Ex Jack) Danser on coffee stems as a natural anticancer. In-silico and in-vitro studies have been carried out on coffee stem parasite extracts to analyze compounds that have the potential to act as HER2 inhibitors, the antioxidant activity of the extract, and the extract's ability to act as an anticancer agent against HeLa and MCF-7 cells. The research results show that several components in coffee stem parasite extract, including flavonoids and fatty acids, have the potential to act as HER2 inhibitors. The coffee stem parasite extract has strong antioxidant activity with IC50 of 59.736 ppm but is inactive against cancer cells. Characterization using gas chromatography-mass spectrometry showed the presence of bis (2-Ethylhexyl) phthalate (C₂₄H₃₈O₄) in coffee stem parasite extract, which is toxic as an anticancer drug.