Insecticidal and Bactericidal activities of Cassia Nigricans and Molecular Docking analysis on Insect Acetylcholinesterase
PDF
Cite
Share
Request
Original Article
P: 0-0

Insecticidal and Bactericidal activities of Cassia Nigricans and Molecular Docking analysis on Insect Acetylcholinesterase

1. University of Ngaoundere, Faculty of Science, Department of Chemistry, Ngaoundere, Cameroon
2. University of Tlemcen, Faculty of Science, Department of Chemistry, Laboratory of Natural and Bioactive Substances, Tlemcen, Algeria
No information available.
No information available
Received Date: 24.07.2023
Accepted Date: 12.01.2024
PDF
Cite
Share
Request

ABSTRACT

Objectives:

This study focused on phytochemicals, insecticidal, and bactericidal activities of Cassia nigricans, as well as the molecular docking analysis for acetylcholinesterase inhibitor (AChEI) as a promising natural insecticide.

Material and methods:

The leaves of C. nigricans were extracted successively with n-hexane, acetone, and methanol. Silica gel column chromatography of the methanol extract yielded compound 1. The insecticidal properties of the extracts and compound 1 were evaluated by contact toxicity against Sitophilus zeamais. The bactericidal activity was achieved by photodynamic inactivation of faecal coliforms and faecal enterococci in water using extracts and compound 1 as natural photosensitizers. Compound 1 was analyzed for physicochemical and pharmacokinetic parameters and molecular docking against an AChE protein (6XYU).

Results:

Compound 1 was characterized as emodin (1,3,8-trihydroxy-6-methylanthracene-9,10-dione) using 1D- 2D-1H-13C NMR and MS methods. The insecticidal properties showed that emodin exhibited the highest toxicity with an LC50 = 5.00 mg/mL compared to all extracts. The n-hexane extract showed the highest insecticidal activity (LC50 = 177.48 mg/mL) compared to the methanol (LC50 = 195.08 mg/mL) and acetone (LC50 = 374.14 mg/mL) extracts. Complete inhibition of faecal enterococci by photosensitization was observed after 60 minutes of light exposure of emodin treated water at all concentrations used (1-5 mg/mL) and 120 minutes for faecal coliforms under the same conditions. Based on the docking score, the binding energy of emodin (-6.38 kcal/mol) is close to that of the marketed insecticide pirimiphos-methyl (-6.25 kcal/mol). In addition, emodin was subjected to insecticide probability prediction and absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis and was found to be satisfactory as a natural insecticide. Emodin could be a promising candidate for insecticidal pest control.