ABSTRACT

In recent years, rııtheniıım (II) comp\exes have increasinglv attracted the interest of researchers because of their high antitumor activities that are usuallv related to DNA binding. Here, we svnthesized two rııtheniıım (II) comp\exes, ıısing imidazo[4,5-fj [l,10]phenanthroline (ip) and 2-phenvlimidazo[4,5-f][flOjphenanthroline (pip) that were characterized bv spectroscopic and elemental analvses. First of ali, we investigated the ability of these complexes to prodııce lethal effects in hum an lııng carcinoma, A549 cells. The cytotoxicity results evaluated bv the MTT assav, revealed that the 1C50 for Ru(ll) complexes of inıidazo[4,5-fj[ 1,10]phenanthroline [Ru(ip)s](PF6)2 and 2-phenvlimidazo[4,5-fj[1,10]phenanthroline [Ru(pip) 3] (PF6) 2 after 24 h of incubation with A549 cells mas approximately 32 and 46 jilg/ml, respectively. İn terestinglv, it mas obsen’ed that these complexes shomed meak cytotoxic effects in normal hum an lııng cells (IC50 > 80 ııg/ml). [Ru(pip)3](PF6)2 has a potent inhibitory effects on A549 compared to other cells as measured bv BrdlJ labeling assav. Treatment of DNA mith [Ru(pip)3](PFğ)3induced DNA binding activitv, mhich mas demonstrated bv a viscosity assav. İn sunimary, Ru(ll) complexes of [Rıı(pip)3](PF6)2 shomed signifıcant cytotoxic and anti-proliferative effects on A549 cells, suggesting that this complex mav have potential therapeııtic agent, and therefore, this must be fıtrther investigated bv other in vitro bioassavs for the developnıent of therapeutic agents.